Protein fractionation achieve highest resolution the agilent 3100 offgel fractionator provides. Stateoftheart user interface for online control of each individual sample. Agilent 33210a product reference cd product software, programming examples, and manuals. Dcaj agilent 86100c widebandwidth oscilloscope mainframe. Agilent automationready cd agilent io libraries suite. Teamwork of the agilent 3100 offgel fractionator, agilent 2100 bioanalyzer and agilent lcms systems. From the myofibrillar extract of each animal sample 4 tough and 4 tender, the volume containing 1 mg of total protein was fractionated by liquid isoelectric focusing using an agilent 3100 offgel fractionator agilent technologies, palo alto, ca, usa following manufacturers instructions.
Individual sample diagnostics for a quick and easy check of fractionation quality with its stateoftheart user interface the 3100 offgel fractionator measures and records. The seven individual proteins were loaded on cm ipg strips ph 3 to 10 by rehydration for 1 hour at ambient temperature, using the tray. To perform peptide fractionation according to their pi, the 3100 offgel. The instrument is highly userfriendly and simple to use. Agilent 6890 service manual university of massachusetts. Agilent 6890 service manual university of massachusetts amherst. Agilent technologies 3100 offgel fractionator with control. Fractionator to fractionate proteins or peptides based on their isoelectric points pi. Agilent 86100c widebandwidth oscilloscope mainframe and modules technical specifications dcaj four instruments in one a digital communications analyzer, a full featured widebandwidth oscilloscope, a timedomain reflectometer, and a jitter analyzer accurate compliance testing of optical transceivers automated jitter and amplitude. This paper illustrates the effectiveness of offgel electrophoresis with the agilent 3100 offgel fractionator for the fractionation of peptides. Offgel user manual appendix ordering information 8 ordering information list of consumables table 6 offgel user manual agilent 3100 offgel fractionator consumable kits product order number product description 3100 offgel, 310, low res kit 51886425 includes frames 12 well, ipgstrips ph 310 cm, cover seals and fractionation reagents. Agilent 4263b lcr meter users guide agilent p art no. The agilent 3100 offgel fractionator uses a novel isoelectric focusing technique to achieve excellent pibased fractionation.
Offgelisoelectric focusing fractionation for the analysis of. Separation of native monoclonal antibodies and identification of charge variants. Agilent 5973 gcms training manual iowa state university. Infilter protein digestion, with easy recovery of a peptide fraction. Manuals or user guides for your hp deskjet 6980 printer. This technical overview demonstrates the ability of the agilent 3100 offgel. Agilent technologies agilent 4155c semiconductor parameter analyzer agilent 4156c. Development of an inhouse protocol for the offgel fractionation of. Individual sample diagnostics for a quick and easy check of fractionation quality with its stateoftheart user interface the 3100 offgel fractionator measures and records voltage, power and current during fractionation in offgel and traditional ief mode. Agilent technologies assumes no liability for the customers failure to comply with these requirements. View and download agilent technologies 3100 offgel fractionator user manual online. Agilent 3100 offgel fractionator user manual agilent technologies 1 product overview intended use 14 features 16 instrument and accessories 17 this chapter gives an overview of the agilent 3100 offgel fractionator.
Adding offgel fractionation into the workflow prior to digestion significantly. Currents of each sample are measured and displayed online. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Agilent 1260 infinity dad and mwd user manual 3 in this guide in this guide this manual covers the agilent 1260 infinity diode array and multiple wavelength detector modules. Instrument state storage with userdefined names portable, ruggedized case with nonskid feet.
Users guide agilent 1100 series handheld control module. Agilent 531a2a 225 mhz universal counter operating guide. Efficient fractionation and improved protein identification by peptide. Using the agilent 3100 offgel fractionator, offgel allows the insolution separation of peptides from various biological sources by isoelectric focusing in up.
Onedimensional proles of the offgel fractions relative to those of. Both the offgel low and high res kit, ph 310 agilent were used. For protein separation, the facility maintains an agilent 3100 offgel fractionator that performs isoelectric focusing based fractionation in solution. Agilent crosslab is a comprehensive, coordinated method of providing services, supplies and software that dramatically improves laboratory. Offgel user manual appendix ordering information 8 ordering information list of consumables table 6 offgel user manual agilent 3100 offgel fractionator consumable kits product order number. Sn for main unit is 91700461 control module sn is cn64400063. Reference guide agilent 81a 400660 mhz pulsedata generator part no.
Service manual agilent model 66332a dynamic measurement dc source and agilent model 6632b, 6633b, 6634b system dc power supply s1 agilent part no. Agilent 1100 hplc g29a autosampler the agilent 1100 hplc g29a autosampler is a variable volume autosampler with a sample capacity of 100 samples. Manual printing history the manuals printing date and part number indicate its current edition. Offgel isoelectric focussing ief has become a popular tool in proteomics to fractionate peptides or proteins. Agilent 3100 offgel fractionator user manual agilent technologies 1 product overview intended use 12 features 14 instrument and accessories 15. We are supportting agilent hp gas chromatograph mass spectrometers models agilent technologies premier laboratory partner for a better world. It describes the intended use and the fracti onation. Agilent 7683b automatic liquid sampler installation, operation and maintenance agilent technologies safety and regulatory information important safety warnings 12 many internal parts of the instrument. Pdf visualisation tool for peptide fractionation data in. To prepare solutions and sample for protein fractionation. General information agilent 6890 series gas chromatograph. Segmented field offgel electrophoresis tobolkina 2012. Systematic comparison of fractionation methods for indepth. The agilent technologies 3100 offgel fractionator with control module uses a novel isoelectric focusing technique to achieve excellent pibased fractionation.
Individual sample diagnostics for a quick and easy check of fractionation quality with its stateoftheart user interface the 3100 offgel fractionator measures and records voltage, power and current during. Protein fractionation, insolution recovery prefractionation of proteins and peptides prior to lcms analysis can dramatically increase the number of proteins ultimately identified. Agilent 1100 hplc g29a autosampler marshall scientific. Warning a warning calls attention to a condition or possible situation that could cause injury to the user. An introduction to using the agilent 54622d digital. The facility offers service to all researchers in the region, with priority given to users from the cmns. Attachments agilent 6890 series gc operation manual 9 mb. Agilent application note finder by part number official. The agilent 3100 offgel fractionator performs ief of proteins in. Ii u1253a users and service guide notices agilent technologies, inc. The electrophoretic separation process was simulated, and optimized by solving the time. Currents of each sample are measured and displayed online individually.
Agilent technologies 3100 offgel fractionator user manual pdf. To look into this further, peptide amino acid structures were manually drawn in. Agilent 3100 offgel fractionator dramatically improve your protein and peptide identification the easytouse agilent 3100 offgel. Conventional isoelectric focusing with the agilent 3100. This book describes how to operate the agilent 1100 series modules and systems for hplc using the control module, shown opposite. Agilent 3100 offgel fractionator the lab world group.
Agilent technologies g23b for agilent technologies 3100. Agilent 7683b automatic liquid sampler installation, operation and maintenance agilent technologies safety and regulatory information important safety warnings 12 many internal parts of the instrument carry dangerous voltages 12 electrostatic discharge is a threat to instrument electronics 12 safety and regulatory certifications information. From the myofibrillar extract of each animal sample 4 tough and 4 tender, the volume containing 1 mg of total protein was fractionated by liquid isoelectric. Agilent technologies 3100 offgel fractionator pdf user manuals. Subproteomic analysis of basic proteins in aged skeletal muscle. The agilent handheld control module selfconfigures all modules for total system control using controllerarea network can communication and cabling. Detergentbased but gelfree method allows identification of several hundred membrane proteins in single lcms runs. Conventional isoelectric focusing with the agilent 3100 offgel. All of the 33210a product documentation is provided on the agilent 33210a product reference cd that comes with the product, and is also. You can control the 4155c4156c using one of the following command modes. Agilent 3100 offgel fractionator user manual agilent technologies 1 product overview intended use 14 features 16 instrument and accessories 17. Caution a caution calls attention to a condition or possible situation that could damage or destroy the product or the users work. This system makes recovery for lcms analysis easier than traditional gels as the resulting fractions are in solution.
Congratulations on having purchased an agilent technologies acqiris data conversion product. Agilent 531a2a 225 mhz universal counter operating. The agilent technologies 33210a is a 10 mhz synthesized function. Offgel fractionation, recently developed by agilent technologies, provides the ability to prefractionate peptides into discrete liquid fractions and demonstrated high efficiency and repeatability. Agilent technologies 3100 offgel fractionator with control module. Offgel fractionation, recently developed by agilent technologies, provides the. This advanced fractionator features a user friendly interface and can connect to a pc or be operated from a smart phone when connected to a. In this manual agilent 4155c4156c provides three command modes to control the 4155c4156c via gpib interface. The agilent technologies g23b is a handheld control module for use in the 3100 series offgel fractionator to provide complete local control and monitoring. As a consequence there is a need for software solutions supporting data mining. Agilent 1260 infinity diode array and multiple wavelength.
Agilent technologies 3100 offgel fractionator forums. See figure 4 in section 1 of the agilent 3100 user manual. Offgel electrophoresis was successfully applied as a prefractionation step. It describes the intended use and the fracti onation principles of the instrument.
Agilent 7683b automatic liquid sampler umass amherst. Agilent 4339b high resistance meter operation manual serial numbers this manual applies directly to instruments with serial number pre x jp1kd, or rmware revision 1. Optimization of itraq labelling coupled to offgel fractionation as. Warranty service may be obtained from the nearest agilent sales office or other location indicated in the owners manual or service booklet. This is a basic introduction to four pieces of equipment listed above. Agilent u1253a true rms oled multimeter users and service guide.
The agilent 3100 offgel fractionator uses a novel isoelectric focusing. Protein isoelectric focusing with the agilent 3100 offgel fractionator. Free download agilent 3100 offgel fractionator manual. Agilent 4155b4156b programmers guide, edition 4 5 programming overview agilent 4155b4156b can be fully controlled from an external computer or by using builtin instrument basic ibasic controller.
Agilent 3100 offgel fractionator used lab equipment. Acqiris instruments are highspeed data acquisition modules designed for capturing high frequency electronic signals. View online or download agilent technologies 3100 offgel fractionator user manual. The control module provides complete local control and monitoring of a single module or an entire agilent 1100 series system. Offgel ief fractionation ogf following the instructions provided with the agilent 3100 offgel fractionator, 1. Results are highly reproducible, and the agilent 3100 offgel fractionator is simple and easy to use. The resulting fractions are in solution, making recovery for lcms analysis much easier than with oldfashioned gels. Agilent 33250a 80 mhz function arbitrary waveform generator. Use of liquid isoelectric focusing offgel on the discovery.
To get the most out of the products we recommend that you read the accompanying product user manual, this programmers guide and. Publication number 3321090001 copyright 2008 agilent. Agilent 4263b lcr meter user s guide agilent p art no. Agilent 531a containing firmware revisions 3317, 3335, or. Agilent 86100c widebandwidth oscilloscope mainframe and modules technical specifications dcaj four instruments in one a digital communications analyzer, a full featured widebandwidth. Agilent 33250a at a glance the agilent technologies 33250a is a highperformance 80 mhz synthesized function generator with builtin arbitrary waveform and pulse capabilities. Prefractionation of proteins and peptides prior to lcms analysis can. Its combination of benchtop and system features makes this function generator a versatile solution for your testing requirements now and in the future. Agilent technologies 3100 offgel fractionator manuals. Proteomics core facility proteomics bioscience cores. The agilent 3100 offgel fractionator provides robust, repeatable insolution fraction recovery that ensures lcms assays are optimized for the maximum number of proteins.